Fast 3D Optoacoustic Mesoscopy of Neuromelanin Through Entire Human Midbrain Organoids at Single‐Cell Resolution

Parkinson's disease is a devastating neurodegenerative disorder characterized by loss of neuromelanin-containing dopaminergic neurons. Novel 3D culture systems like human midbrain-like organoids (hMOs) enable new research avenues for patient-specific therapies, but cannot reach their full potential unless rapid optical imaging entire enabled. Currently, hMOs have to undergo tissue clearing processes before overcome light scattering. Since lengthy chemical procedure, large ensemble studies and pharmacological longitudinal studies, which require live cultures, are impossible. To address this obstacle, raster scanning optoacoustic mesoscopy (RSOM) considered intact hMOs. RSOM an technique that leverages the effect need clearing. Moreover, using tomographic principles, specimens can be imaged within minutes. The results confirm image neuromelanin distribution in complete at single-cell resolution. Whole hMO volumes standard size 4 min. Comparison with bright-field microscopy histology confirms ground truth images. This work opens several opportunities regarding boost Parkinson disease.